Docker config
This commit is contained in:
@ -0,0 +1,20 @@
|
||||
// CircularAdvantages.jsx
|
||||
import AdvantagesLayout from '../../../components/shared/AdvantagesLayout';
|
||||
|
||||
const CircularAdvantages = () => {
|
||||
const advantageItems = [
|
||||
"Circular RNAs are inherently more stable due to their covalently closed structure, making them less susceptible to degradation and providing reliable expression data.",
|
||||
"Enables the identification of novel isoforms and splice variants that may not be detected with traditional RNA sequencing methods.",
|
||||
"Offers a detailed view of circular RNA expression and their interactions within the cell, aiding in a deeper understanding of gene regulation and cellular processes.",
|
||||
"Closed-loop structure of circRNAs minimizes potential sequencing artifacts, leading to more accurate and consistent data."
|
||||
];
|
||||
|
||||
return (
|
||||
<AdvantagesLayout
|
||||
title="Advantages of Circular RNA Sequencing"
|
||||
advantageItems={advantageItems}
|
||||
/>
|
||||
);
|
||||
};
|
||||
|
||||
export default CircularAdvantages;
|
||||
@ -0,0 +1,21 @@
|
||||
// CircularApplications.jsx
|
||||
import ApplicationsLayout from '../../../components/shared/ApplicationsLayout';
|
||||
|
||||
const CircularApplications = () => {
|
||||
const applicationItems = [
|
||||
"Circular RNA sequencing is widely utilized across plants, animals, and humans.",
|
||||
"Therapeutic Targets for Cancer- Small molecules or antisense oligonucleotides can be used to modulate circRNA activity. Consequently, targeting oncogenic circRNAs could provide novel strategies for inhibiting tumor growth.",
|
||||
"Developmental Biology- Investigates circRNA roles in developmental processes and differentiation, enhancing understanding of gene regulation during growth and development.",
|
||||
"Disease Biomarkers- circRNAs are stable in body fluids such as blood and saliva, and exhibit altered expression in conditions like cancer, cardiovascular disorders, and neurological diseases. This stability and variation in expression patterns suggest their potential as biomarkers for these diseases.",
|
||||
"Functional Studies- Functional studies can involve manipulating circRNA levels in cell models through techniques such as knockdown or CRISPR/Cas9, to investigate their roles in cellular processes."
|
||||
];
|
||||
|
||||
return (
|
||||
<ApplicationsLayout
|
||||
title="Applications of Circular RNA Sequencing"
|
||||
applicationItems={applicationItems}
|
||||
/>
|
||||
);
|
||||
};
|
||||
|
||||
export default CircularApplications;
|
||||
@ -0,0 +1,22 @@
|
||||
// app/rna-sequencing/circular-rna-sequencing/components/CircularIntroduction.jsx
|
||||
import IntroductionLayout from '../../../components/shared/IntroductionLayout';
|
||||
|
||||
const CircularIntroduction = () => {
|
||||
const contentItems = [
|
||||
"Circular RNA sequencing (circRNA-seq) focuses on identifying and characterizing non-coding circular RNAs formed through back-splicing events. These circRNAs regulate gene expression by acting as miRNA sponges, influence alternative splicing, modulate transcription, interact with proteins, and serve as potential biomarkers and therapeutic targets.",
|
||||
"As compared to Linear RNAs, circRNAs are covalently closed loops with no free ends, which provides them with enhanced stability and distinct regulatory functions in gene expression and cellular processes.",
|
||||
"The workflow starts with isolating total RNA from the sample, which includes both linear and circular RNAs. Linear RNAs are then removed through ribosomal RNA (rRNA) depletion or exonuclease treatment to enrich the circular RNA fraction. Next, the RNA is circularized, and sequencing adapters are added during library preparation. The final step involves sequencing the prepared libraries.",
|
||||
"Bioinformatics analysis for circRNA sequencing involves preprocessing data and mapping reads to a reference genome to identify back-splicing junctions. Computational tools are then used to quantify circRNA expression, discover novel circRNAs, and perform downstream analysis."
|
||||
];
|
||||
|
||||
return (
|
||||
<IntroductionLayout
|
||||
title="Introduction and Workflow"
|
||||
contentItems={contentItems}
|
||||
badgeSubtext="image from Circular RNA Sequencing"
|
||||
useParagraphs={true}
|
||||
/>
|
||||
);
|
||||
};
|
||||
|
||||
export default CircularIntroduction;
|
||||
@ -0,0 +1,57 @@
|
||||
// app/rna-sequencing/circular-rna-sequencing/components/CircularSpecifications.jsx
|
||||
import Link from 'next/link';
|
||||
import SpecificationsLayout from '../../../components/shared/SpecificationsLayout';
|
||||
|
||||
const CircularSpecifications = () => {
|
||||
const specificationItems = [
|
||||
{
|
||||
icon: "/images/homepage-2/NGS-Icons-45.svg",
|
||||
title: "Sample Requirement",
|
||||
renderContent: () => (
|
||||
<div>
|
||||
<div className="mb-4">
|
||||
<p className="text-gray-600">Total RNA amount: ≥ 2.0 μg, Blood and other samples</p>
|
||||
</div>
|
||||
<div className="mt-4 text-sm">
|
||||
<strong>
|
||||
Please refer to{' '}
|
||||
<Link href="/sample-submission-guidelines" className="text-teal-600 underline hover:text-teal-700">
|
||||
sample submission guidelines
|
||||
</Link>
|
||||
{' '}or{' '}
|
||||
<Link href="/contact" className="text-teal-600 underline hover:text-teal-700">
|
||||
Contact Us!
|
||||
</Link>
|
||||
</strong>
|
||||
</div>
|
||||
</div>
|
||||
)
|
||||
},
|
||||
{
|
||||
icon: "/images/homepage-1/service/Advantages-NGS-Icons-20.svg",
|
||||
title: "Sequencing Platform",
|
||||
content: "Illumina NovaSeq 6000/ NovaSeq X"
|
||||
},
|
||||
{
|
||||
icon: "/images/service/social-support.png",
|
||||
title: "Deliverables",
|
||||
renderContent: () => (
|
||||
<ul className="list-disc pl-5 space-y-2 text-gray-600 text-start">
|
||||
<li>The original sequencing data</li>
|
||||
<li>Experimental results</li>
|
||||
<li>Bioinformatics and Data Analysis Report</li>
|
||||
<li>Details of Circular RNA Sequencing (customizable)</li>
|
||||
</ul>
|
||||
)
|
||||
}
|
||||
];
|
||||
|
||||
return (
|
||||
<SpecificationsLayout
|
||||
title="Service Specifications"
|
||||
specificationItems={specificationItems}
|
||||
/>
|
||||
);
|
||||
};
|
||||
|
||||
export default CircularSpecifications;
|
||||
27
app/rna-sequencing/circular-rna-sequencing/page.js
Normal file
27
app/rna-sequencing/circular-rna-sequencing/page.js
Normal file
@ -0,0 +1,27 @@
|
||||
import TitleBar from '../../components/shared/TitleBar';
|
||||
import PageLayout from '../../components/Layout/PageLayout'
|
||||
import CircularIntroduction from './components/CircularIntroduction';
|
||||
import CircularAdvantages from './components/CircularAdvantages';
|
||||
import CircularApplications from './components/CircularApplications';
|
||||
import CircularSpecifications from './components/CircularSpecifications';
|
||||
|
||||
export default function CircularRNASequencingPage() {
|
||||
const breadcrumbs = [
|
||||
{ label: 'Home', href: '/', current: false },
|
||||
{ label: 'RNA Sequencing', href: '/rna-sequencing', current: false },
|
||||
{ label: 'Circular RNA Sequencing', href: '#', current: true }
|
||||
];
|
||||
|
||||
return (
|
||||
<PageLayout fixedHeader={true}>
|
||||
<TitleBar
|
||||
title="Circular RNA Sequencing"
|
||||
breadcrumbs={breadcrumbs}
|
||||
/>
|
||||
<CircularIntroduction />
|
||||
<CircularAdvantages />
|
||||
<CircularApplications />
|
||||
<CircularSpecifications />
|
||||
</PageLayout>
|
||||
);
|
||||
}
|
||||
@ -0,0 +1,20 @@
|
||||
// DegradomeAdvantages.jsx
|
||||
import AdvantagesLayout from '../../../components/shared/AdvantagesLayout';
|
||||
|
||||
const DegradomeAdvantages = () => {
|
||||
const advantageItems = [
|
||||
"Degradome sequencing provides a focused analysis of RNA degradation, specifically targeting cleavage sites mediated by miRNAs and ta-siRNAs, making it ideal for studying gene regulation and RNA stability.",
|
||||
"By targeting degradation products, degradome sequencing minimizes the impact of intact RNAs, enhancing sensitivity for detecting miRNA targets and cleavage events.",
|
||||
"Degradome sequencing focuses on RNA cleavage sites, eliminating the need for additional steps like ribosomal RNA removal, making it a more cost-effective alternative to broader transcriptome studies.",
|
||||
"Degradome sequencing enables the identification of novel regulatory elements, such as circRNAs and biomarkers, expanding its utility in understanding RNA regulatory networks."
|
||||
];
|
||||
|
||||
return (
|
||||
<AdvantagesLayout
|
||||
title="Advantages of Degradome Sequencing"
|
||||
advantageItems={advantageItems}
|
||||
/>
|
||||
);
|
||||
};
|
||||
|
||||
export default DegradomeAdvantages;
|
||||
@ -0,0 +1,22 @@
|
||||
// DegradomeApplications.jsx
|
||||
import ApplicationsLayout from '../../../components/shared/ApplicationsLayout';
|
||||
|
||||
const DegradomeApplications = () => {
|
||||
const applicationItems = [
|
||||
"Gene Regulation Studies- Used to identify miRNA targets and cleavage sites, elucidating the regulatory networks that control gene expression and RNA turnover in different organisms.",
|
||||
"Developmental Biology- Explores RNA degradation patterns during developmental stages, uncovering the molecular mechanisms underlying cell differentiation and tissue formation.",
|
||||
"Disease Research- Investigates RNA cleavage profiles associated with diseases such as cancer and neurodegenerative disorders, helping to identify potential biomarkers and therapeutic targets.",
|
||||
"Functional Genomics- Provides a detailed view of RNA decay dynamics, enabling the study of gene function and the roles of regulatory RNAs in cellular processes.",
|
||||
"Comparative Genomics- Analyzes RNA degradation patterns across species or environmental conditions to understand evolutionary conservation and differences in regulatory mechanisms.",
|
||||
"Stress and Environmental Responses- Examines changes in RNA stability under stress conditions, shedding light on the molecular mechanisms of stress adaptation and survival strategies."
|
||||
];
|
||||
|
||||
return (
|
||||
<ApplicationsLayout
|
||||
title="Applications of Degradome Sequencing"
|
||||
applicationItems={applicationItems}
|
||||
/>
|
||||
);
|
||||
};
|
||||
|
||||
export default DegradomeApplications;
|
||||
@ -0,0 +1,22 @@
|
||||
// app/rna-sequencing/degradome-sequencing/components/DegradomeIntroduction.jsx
|
||||
import IntroductionLayout from '../../../components/shared/IntroductionLayout';
|
||||
|
||||
const DegradomeIntroduction = () => {
|
||||
const contentItems = [
|
||||
"Degradome sequencing, also called Parallel Analysis of RNA Ends (PARE), is a high-throughput technique to analyze RNA degradation products, uncovering RNA stability, miRNA targets, and regulatory networks.",
|
||||
"Degradome Sequencing Workflow combines next-generation sequencing (NGS) with a modified 5' RACE method, involving RNA extraction, adapter ligation to capture degradation products, cDNA synthesis, and high-throughput sequencing to map RNA cleavage sites.",
|
||||
"Bioinformatics analysis for Degradome Sequencing include data preprocessing, aligning reads to reference genomes, identifying miRNA/ta-siRNA cleavage sites, and quantifying cleaved targets, revealing RNA decay dynamics and regulatory mechanisms.",
|
||||
"The method facilitates the discovery of novel miRNAs and ta-siRNAs, studies on miRNA-mediated gene regulation, and comparative RNA decay analysis across species, contributing to a comprehensive understanding of RNA life cycles."
|
||||
];
|
||||
|
||||
return (
|
||||
<IntroductionLayout
|
||||
title="Introduction and Workflow"
|
||||
contentItems={contentItems}
|
||||
badgeSubtext="Brochure from Degradome sequencing"
|
||||
useParagraphs={true}
|
||||
/>
|
||||
);
|
||||
};
|
||||
|
||||
export default DegradomeIntroduction;
|
||||
@ -0,0 +1,58 @@
|
||||
// DegradomeSpecifications.jsx
|
||||
import Link from 'next/link';
|
||||
import SpecificationsLayout from '../../../components/shared/SpecificationsLayout';
|
||||
|
||||
const DegradomeSpecifications = () => {
|
||||
const specificationItems = [
|
||||
{
|
||||
icon: "/images/homepage-2/NGS-Icons-45.svg",
|
||||
title: "Sample Requirement",
|
||||
renderContent: () => (
|
||||
<div>
|
||||
<div className="mb-4">
|
||||
<p className="text-gray-600">Sample type: Total RNA without degradation or DNA contamination</p>
|
||||
<p className="text-gray-600">Total RNA≥ 20 μg, Minimum Quantity: 15 μg, Concentration≥ 100 ng/µL</p>
|
||||
</div>
|
||||
<div className="mt-4 text-sm">
|
||||
<strong>
|
||||
Please refer to{' '}
|
||||
<Link href="/sample-submission-guidelines" className="text-teal-600 underline hover:text-teal-700">
|
||||
sample submission guidelines
|
||||
</Link>
|
||||
{' '}or{' '}
|
||||
<Link href="/contact" className="text-teal-600 underline hover:text-teal-700">
|
||||
Contact Us!
|
||||
</Link>
|
||||
</strong>
|
||||
</div>
|
||||
</div>
|
||||
)
|
||||
},
|
||||
{
|
||||
icon: "/images/homepage-1/service/Advantages-NGS-Icons-20.svg",
|
||||
title: "Sequencing Platform",
|
||||
content: "Illumina NovaSeq 6000/ NovaSeq X"
|
||||
},
|
||||
{
|
||||
icon: "/images/service/social-support.png",
|
||||
title: "Deliverables",
|
||||
renderContent: () => (
|
||||
<ul className="list-disc pl-5 space-y-2 text-gray-600 text-start">
|
||||
<li>The original sequencing data</li>
|
||||
<li>Experimental results</li>
|
||||
<li>Bioinformatics and Data analysis report</li>
|
||||
<li>Details of Degradome Sequencing (customizable)</li>
|
||||
</ul>
|
||||
)
|
||||
}
|
||||
];
|
||||
|
||||
return (
|
||||
<SpecificationsLayout
|
||||
title="Service Specifications"
|
||||
specificationItems={specificationItems}
|
||||
/>
|
||||
);
|
||||
};
|
||||
|
||||
export default DegradomeSpecifications;
|
||||
27
app/rna-sequencing/degradome-sequencing/page.js
Normal file
27
app/rna-sequencing/degradome-sequencing/page.js
Normal file
@ -0,0 +1,27 @@
|
||||
import TitleBar from '../../components/shared/TitleBar';
|
||||
import DegradomeIntroduction from './components/DegradomeIntroduction';
|
||||
import DegradomeAdvantages from './components/DegradomeAdvantages';
|
||||
import DegradomeApplications from './components/DegradomeApplications';
|
||||
import DegradomeSpecifications from './components/DegradomeSpecifications';
|
||||
import PageLayout from '../../components/Layout/PageLayout';
|
||||
|
||||
export default function DegradomeSequencingPage() {
|
||||
const breadcrumbs = [
|
||||
{ label: 'Home', href: '/', current: false },
|
||||
{ label: 'RNA Sequencing', href: '/rna-sequencing', current: false },
|
||||
{ label: 'Degradome Sequencing', href: '#', current: true }
|
||||
];
|
||||
|
||||
return (
|
||||
<PageLayout fixedHeader={true}>
|
||||
<TitleBar
|
||||
title="Degradome Sequencing"
|
||||
breadcrumbs={breadcrumbs}
|
||||
/>
|
||||
<DegradomeIntroduction />
|
||||
<DegradomeAdvantages />
|
||||
<DegradomeApplications />
|
||||
<DegradomeSpecifications />
|
||||
</PageLayout>
|
||||
);
|
||||
}
|
||||
@ -0,0 +1,20 @@
|
||||
// IsoAdvantages.jsx
|
||||
import AdvantagesLayout from '../../../components/shared/AdvantagesLayout';
|
||||
|
||||
const IsoAdvantages = () => {
|
||||
const advantageItems = [
|
||||
"Unlike traditional methods, which may miss complex isoforms or splice variants, Iso-Seq captures full-length RNA sequences, enabling the identification of all transcript isoforms, including those with complex splicing and alternative start or end sites.",
|
||||
"Provides high-fidelity data with long read lengths, reducing errors and missing information common in shorter-read sequencing methods.",
|
||||
"Reduces challenges with transcript assembly and alignment by providing full-length reads that eliminate ambiguities and enhance transcriptome reconstruction accuracy. Improves genome annotations by precisely identifying gene structures, exons, introns, and regulatory elements, leading to more accurate functional insights.",
|
||||
"Uncovers novel isoforms, splice variants, and post-transcriptional modifications, providing a comprehensive view of gene expression. This detailed approach is essential for functional genomics, transcriptomics, and alternative splicing studies, offering valuable insights into gene regulation and function."
|
||||
];
|
||||
|
||||
return (
|
||||
<AdvantagesLayout
|
||||
title="Advantages of Isoform Sequencing"
|
||||
advantageItems={advantageItems}
|
||||
/>
|
||||
);
|
||||
};
|
||||
|
||||
export default IsoAdvantages;
|
||||
@ -0,0 +1,23 @@
|
||||
// IsoApplications.jsx
|
||||
import ApplicationsLayout from '../../../components/shared/ApplicationsLayout';
|
||||
|
||||
const IsoApplications = () => {
|
||||
const applicationItems = [
|
||||
"Isoform sequencing is widely utilized across plants, animals, microbes, and humans.",
|
||||
"Gene Annotation- Enhances the annotation of complex genomes by providing precise information on transcript structures, helping to accurately define gene boundaries and functions.",
|
||||
"Alternative Splicing Analysis- Investigates alternative splicing events and isoform diversity, contributing to a deeper understanding of gene regulation and functional diversity.",
|
||||
"Functional Genomics- Supports functional studies by linking isoform-specific expression profiles to phenotypic traits, disease mechanisms, and developmental processes.",
|
||||
"Disease Research- Identifies aberrant isoforms associated with diseases, aiding in the discovery of biomarkers and therapeutic targets for conditions such as cancer and genetic disorders.",
|
||||
"Plant and Crop Improvement- It allows the haplotype resolution of complex polyploids like those in plants. Explores transcript diversity in plants and crops, contributing to better understanding of stress responses, developmental processes, and traits relevant for agriculture.",
|
||||
"Microbial Genomics- Studies microbial transcriptomes to uncover complex regulatory networks, metabolic pathways, and interactions within microbial communities, advancing our knowledge in microbiology and biotechnology."
|
||||
];
|
||||
|
||||
return (
|
||||
<ApplicationsLayout
|
||||
title="Applications of Isoform Sequencing"
|
||||
applicationItems={applicationItems}
|
||||
/>
|
||||
);
|
||||
};
|
||||
|
||||
export default IsoApplications;
|
||||
@ -0,0 +1,23 @@
|
||||
// app/rna-sequencing/iso-sequencing/components/IsoIntroduction.jsx
|
||||
import IntroductionLayout from '../../../components/shared/IntroductionLayout';
|
||||
|
||||
const IsoIntroduction = () => {
|
||||
const contentItems = [
|
||||
"Iso-Seq, or Isoform Sequencing, leverages PCR free PacBio's Single Molecule, Real-Time (SMRT) sequencing technology to deliver detailed and accurate insights into RNA transcripts. PCR free SMRT technology produces long reads of upto 25Mbps length with 99.9% base level accuracy through circular consensus sequencing (CCS) mode.",
|
||||
"PacBio's SMRT sequencing technology enables the reading of full-length cDNA molecules in a single pass, allowing for the accurate identification of various isoforms, including alternative splicing events and post-transcriptional modifications. This approach minimizes errors and provides a high-resolution map of transcript structures.",
|
||||
"The process begins with the isolation of high-quality RNA, which is then converted into cDNA followed by sequencing using PacBio SMRT technology.",
|
||||
"The bioinformatics analysis of Iso-Seq data involves assembling full-length transcripts, annotating them, and comparing them to reference genomes or existing databases. This detailed analysis reveals the complexity of gene expression, including novel isoforms and splice variants, and aids in understanding their functional roles in various biological processes.",
|
||||
"Iso-Seq using PacBio is particularly valuable for exploring gene expression at a granular level, discovering novel transcripts, and enhancing annotations of complex genomes."
|
||||
];
|
||||
|
||||
return (
|
||||
<IntroductionLayout
|
||||
title="Introduction and Workflow"
|
||||
contentItems={contentItems}
|
||||
badgeSubtext="image from Iso Sequencing using PacBio"
|
||||
useParagraphs={true}
|
||||
/>
|
||||
);
|
||||
};
|
||||
|
||||
export default IsoIntroduction;
|
||||
@ -0,0 +1,57 @@
|
||||
// IsoSpecifications.jsx
|
||||
import Link from 'next/link';
|
||||
import SpecificationsLayout from '../../../components/shared/SpecificationsLayout';
|
||||
|
||||
const IsoSpecifications = () => {
|
||||
const specificationItems = [
|
||||
{
|
||||
icon: "/images/homepage-2/NGS-Icons-45.svg",
|
||||
title: "Sample Requirement",
|
||||
renderContent: () => (
|
||||
<div>
|
||||
<div className="mb-4">
|
||||
<p className="text-gray-600">Frozen cells in specific frozen lysates, High Quality RNA</p>
|
||||
</div>
|
||||
<div className="mt-4 text-sm">
|
||||
<strong>
|
||||
Please refer to{' '}
|
||||
<Link href="/sample-submission-guidelines" className="text-teal-600 underline hover:text-teal-700">
|
||||
sample submission guidelines
|
||||
</Link>
|
||||
{' '}or{' '}
|
||||
<Link href="/contact" className="text-teal-600 underline hover:text-teal-700">
|
||||
Contact Us!
|
||||
</Link>
|
||||
</strong>
|
||||
</div>
|
||||
</div>
|
||||
)
|
||||
},
|
||||
{
|
||||
icon: "/images/homepage-1/service/Advantages-NGS-Icons-20.svg",
|
||||
title: "Sequencing Platform",
|
||||
content: "PacBio"
|
||||
},
|
||||
{
|
||||
icon: "/images/service/social-support.png",
|
||||
title: "Deliverables",
|
||||
renderContent: () => (
|
||||
<ul className="list-disc pl-5 space-y-2 text-gray-600 text-start">
|
||||
<li>The original sequencing data</li>
|
||||
<li>Experimental results</li>
|
||||
<li>Bioinformatics and Data analysis report</li>
|
||||
<li>Details of Iso-Seq Sequencing (customizable)</li>
|
||||
</ul>
|
||||
)
|
||||
}
|
||||
];
|
||||
|
||||
return (
|
||||
<SpecificationsLayout
|
||||
title="Service Specifications"
|
||||
specificationItems={specificationItems}
|
||||
/>
|
||||
);
|
||||
};
|
||||
|
||||
export default IsoSpecifications;
|
||||
27
app/rna-sequencing/iso-sequencing/page.js
Normal file
27
app/rna-sequencing/iso-sequencing/page.js
Normal file
@ -0,0 +1,27 @@
|
||||
import TitleBar from '../../components/shared/TitleBar';
|
||||
import IsoIntroduction from './components/IsoIntroduction';
|
||||
import IsoAdvantages from './components/IsoAdvantages';
|
||||
import IsoApplications from './components/IsoApplications';
|
||||
import IsoSpecifications from './components/IsoSpecifications';
|
||||
import PageLayout from '../../components/Layout/PageLayout';
|
||||
|
||||
export default function IsoSequencingPage() {
|
||||
const breadcrumbs = [
|
||||
{ label: 'Home', href: '/', current: false },
|
||||
{ label: 'RNA Sequencing', href: '/rna-sequencing', current: false },
|
||||
{ label: 'Iso Sequencing using PacBio', href: '#', current: true }
|
||||
];
|
||||
|
||||
return (
|
||||
<PageLayout fixedHeader={true}>
|
||||
<TitleBar
|
||||
title="Iso Sequencing using PacBio"
|
||||
breadcrumbs={breadcrumbs}
|
||||
/>
|
||||
<IsoIntroduction />
|
||||
<IsoAdvantages />
|
||||
<IsoApplications />
|
||||
<IsoSpecifications />
|
||||
</PageLayout>
|
||||
);
|
||||
}
|
||||
@ -0,0 +1,20 @@
|
||||
// LncRNAAdvantages.jsx
|
||||
import AdvantagesLayout from '../../../components/shared/AdvantagesLayout';
|
||||
|
||||
const LncRNAAdvantages = () => {
|
||||
const advantageItems = [
|
||||
"Identifies both well-known and novel lncRNAs, providing fresh insights into their roles and potential as biomarkers.",
|
||||
"Reveals lncRNAs' involvement in a broad range of molecular functions, such as chromatin remodeling, which is essential for regulating gene expression. This process includes modifying chromatin structure to control gene accessibility and influencing various cellular mechanisms, demonstrating lncRNAs' crucial roles in gene regulation and cellular function across mammals and plants.",
|
||||
"Provides a thorough understanding of lncRNAs' roles in gene regulation, encompassing both transcriptional and post-transcriptional levels. It highlights how lncRNAs affect transcription factors, alter nucleosome positioning, and contribute to RNA splicing and stability, thus impacting overall gene expression and cellular processes.",
|
||||
"Enables high-resolution analysis, allowing for precise detection and detailed characterization of lncRNA expression and their regulatory networks, offering a deep dive into their functional roles within cells."
|
||||
];
|
||||
|
||||
return (
|
||||
<AdvantagesLayout
|
||||
title="Advantages of lncRNA sequencing"
|
||||
advantageItems={advantageItems}
|
||||
/>
|
||||
);
|
||||
};
|
||||
|
||||
export default LncRNAAdvantages;
|
||||
@ -0,0 +1,24 @@
|
||||
// LncRNAApplications.jsx
|
||||
import ApplicationsLayout from '../../../components/shared/ApplicationsLayout';
|
||||
|
||||
const LncRNAApplications = () => {
|
||||
const applicationItems = [
|
||||
"LncRNA sequencing is widely utilized across plants, animals, microbes, and humans.",
|
||||
"Cancer Research- Identifies tumor-specific lncRNAs and their roles in cancer development, progression, and metastasis, offering potential biomarkers and therapeutic targets.",
|
||||
"Developmental Biology- Studies lncRNAs involved in developmental processes and cell differentiation, providing insights into gene regulation during development.",
|
||||
"Disease Mechanisms- Uncovers lncRNAs associated with various diseases, including genetic disorders, cardiovascular diseases, and neurodegenerative conditions, facilitating the discovery of novel diagnostic and prognostic markers.",
|
||||
"Drug Discovery- Assists in identifying lncRNAs that can be targeted or modulated by drugs, contributing to the development of new therapeutic strategies.",
|
||||
"Gene Regulation- Explores lncRNAs' roles in regulating gene expression through chromatin remodeling, transcriptional control, and post-transcriptional modifications, enhancing the understanding of complex gene regulatory networks.",
|
||||
"Functional Genomics- Provides insights into the functional roles of lncRNAs in cellular processes, helping to elucidate their impact on gene expression and cellular functions.",
|
||||
"Comparative Genomics- Analyzes lncRNA profiles across different species or conditions, supporting research into evolutionary differences and functional conservation of lncRNAs."
|
||||
];
|
||||
|
||||
return (
|
||||
<ApplicationsLayout
|
||||
title="Applications of lncRNA Sequencing"
|
||||
applicationItems={applicationItems}
|
||||
/>
|
||||
);
|
||||
};
|
||||
|
||||
export default LncRNAApplications;
|
||||
@ -0,0 +1,33 @@
|
||||
// app/rna-sequencing/lncrna-sequencing/components/LncRNABioinformatics.jsx
|
||||
|
||||
const LncRNABioinformatics = () => {
|
||||
return (
|
||||
<section className="py-8 lg:py-12 bg-gray-50">
|
||||
<div className="container mx-auto max-w-none px-4 lg:px-6">
|
||||
<h2 className="text-2xl lg:text-3xl text-gray-700 text-left pb-2 mb-6 lg:mb-6">
|
||||
Bioinformatics Pipeline
|
||||
</h2>
|
||||
|
||||
{/* Pipeline Image */}
|
||||
<div className="bg-white rounded-xl shadow-lg p-6 lg:p-8">
|
||||
<div className="flex justify-center">
|
||||
<img
|
||||
src="/images/lncrna-bioinformatics-pipeline.jpg"
|
||||
alt="lncRNA Sequencing Bioinformatics Pipeline Workflow"
|
||||
className="max-w-full h-auto rounded-lg"
|
||||
/>
|
||||
</div>
|
||||
|
||||
{/* Pipeline Description */}
|
||||
<div className="mt-6 text-center">
|
||||
<p className="text-gray-600 text-sm lg:text-base">
|
||||
lncRNA sequencing bioinformatics pipeline for long non-coding RNA analysis and expression profiling
|
||||
</p>
|
||||
</div>
|
||||
</div>
|
||||
</div>
|
||||
</section>
|
||||
);
|
||||
};
|
||||
|
||||
export default LncRNABioinformatics
|
||||
@ -0,0 +1,22 @@
|
||||
// app/rna-sequencing/lncrna-sequencing/components/LncRNAIntroduction.jsx
|
||||
import IntroductionLayout from '../../../components/shared/IntroductionLayout';
|
||||
|
||||
const LncRNAIntroduction = () => {
|
||||
const contentItems = [
|
||||
"Long non-coding RNAs (lncRNAs) are a class of RNA molecules longer than 200 nucleotides that do not code for proteins. Based on their position and direction of transcription, lncRNAs are further classified into subtypes such as antisense, intergenic, overlapping, intronic, bidirectional, and processed. These RNAs play essential roles in regulating gene expression at various levels, impacting a wide range of cellular processes.",
|
||||
"lncRNA sequencing workflow starts with the removal of ribosomal RNA (rRNA) from the total RNA sample to isolate lncRNAs and mRNAs. The isolated RNA is then subjected to library preparation and high-throughput sequencing. Through specific computational pipelines lncRNAs will be identified during bio-informatic analysis.",
|
||||
"The bioinformatics pipeline for lncRNA sequencing involves the alignment of sequencing data to reference genomes, identification of lncRNAs, quantification of their expression level, and other downstream analyses.",
|
||||
"lncRNA sequencing uncovers their roles in gene regulation and highlights their various functions, including chromatin remodeling, transcriptional control, nucleosome repositioning, and post-transcriptional processing."
|
||||
];
|
||||
|
||||
return (
|
||||
<IntroductionLayout
|
||||
title="Introduction and Workflow"
|
||||
contentItems={contentItems}
|
||||
badgeSubtext="Brochure lncRNA Sequencing"
|
||||
useParagraphs={true}
|
||||
/>
|
||||
);
|
||||
};
|
||||
|
||||
export default LncRNAIntroduction;
|
||||
@ -0,0 +1,57 @@
|
||||
// LncRNASpecifications.jsx
|
||||
import Link from 'next/link';
|
||||
import SpecificationsLayout from '../../../components/shared/SpecificationsLayout';
|
||||
|
||||
const LncRNASpecifications = () => {
|
||||
const specificationItems = [
|
||||
{
|
||||
icon: "/images/homepage-2/NGS-Icons-45.svg",
|
||||
title: "Sample Requirement",
|
||||
renderContent: () => (
|
||||
<div>
|
||||
<div className="mb-4">
|
||||
<p className="text-gray-600">Total RNA ≥ 1 μg, Cells≥ 2×10<sup>6</sup>, or Tissue ≥ 500 mg.</p>
|
||||
</div>
|
||||
<div className="mt-4 text-sm">
|
||||
<strong>
|
||||
Please refer to{' '}
|
||||
<Link href="/sample-submission-guidelines" className="text-teal-600 underline hover:text-teal-700">
|
||||
sample submission guidelines
|
||||
</Link>
|
||||
{' '}or{' '}
|
||||
<Link href="/contact" className="text-teal-600 underline hover:text-teal-700">
|
||||
Contact Us!
|
||||
</Link>
|
||||
</strong>
|
||||
</div>
|
||||
</div>
|
||||
)
|
||||
},
|
||||
{
|
||||
icon: "/images/homepage-1/service/Advantages-NGS-Icons-20.svg",
|
||||
title: "Sequencing Platform",
|
||||
content: "Illumina NovaSeq 6000/ NovaSeq X"
|
||||
},
|
||||
{
|
||||
icon: "/images/service/social-support.png",
|
||||
title: "Deliverables",
|
||||
renderContent: () => (
|
||||
<ul className="list-disc pl-5 space-y-2 text-gray-600 text-start">
|
||||
<li>The original sequencing data</li>
|
||||
<li>Experimental results</li>
|
||||
<li>Bioinformatics and Data analysis report</li>
|
||||
<li>Details of LncRNA Sequencing (customizable)</li>
|
||||
</ul>
|
||||
)
|
||||
}
|
||||
];
|
||||
|
||||
return (
|
||||
<SpecificationsLayout
|
||||
title="Service Specifications"
|
||||
specificationItems={specificationItems}
|
||||
/>
|
||||
);
|
||||
};
|
||||
|
||||
export default LncRNASpecifications;
|
||||
30
app/rna-sequencing/lncrna-sequencing/page.js
Normal file
30
app/rna-sequencing/lncrna-sequencing/page.js
Normal file
@ -0,0 +1,30 @@
|
||||
// app/rna-sequencing/lncrna-sequencing/page.js
|
||||
import TitleBar from '../../components/shared/TitleBar';
|
||||
import LncRNAIntroduction from './components/LncRNAIntroduction';
|
||||
import LncRNAAdvantages from './components/LncRNAAdvantages';
|
||||
import LncRNABioinformatics from './components/LncRNABioinformatics';
|
||||
import LncRNAApplications from './components/LncRNAApplications';
|
||||
import LncRNASpecifications from './components/LncRNASpecifications';
|
||||
import PageLayout from '../../components/Layout/PageLayout';
|
||||
|
||||
export default function LncRNASequencingPage() {
|
||||
const breadcrumbs = [
|
||||
{ label: 'Home', href: '/', current: false },
|
||||
{ label: 'RNA Sequencing', href: '/rna-sequencing', current: false },
|
||||
{ label: 'lncRNA Sequencing', href: '#', current: true }
|
||||
];
|
||||
|
||||
return (
|
||||
<PageLayout fixedHeader={true}>
|
||||
<TitleBar
|
||||
title="LncRNA Sequencing"
|
||||
breadcrumbs={breadcrumbs}
|
||||
/>
|
||||
<LncRNAIntroduction />
|
||||
<LncRNAAdvantages />
|
||||
<LncRNABioinformatics />
|
||||
<LncRNAApplications />
|
||||
<LncRNASpecifications />
|
||||
</PageLayout>
|
||||
);
|
||||
}
|
||||
@ -0,0 +1,20 @@
|
||||
// MetatranscriptomicsAdvantages.jsx
|
||||
import AdvantagesLayout from '../../../components/shared/AdvantagesLayout';
|
||||
|
||||
const MetatranscriptomicsAdvantages = () => {
|
||||
const advantageItems = [
|
||||
"Provides insights into the functional dynamics of microorganisms by revealing active gene expression and their roles within complex communities. This approach uncovers how microorganisms contribute to ecosystem processes and their interactions.",
|
||||
"Analyzes microbial communities directly from environmental samples, removing the need for isolating or cultivating individual microbes. This method captures a more comprehensive view of microbial diversity.",
|
||||
"Uses next-generation sequencing (NGS) to identify novel genes and track transcriptional changes, offering high-resolution data on gene expression. This allows for precise identification of active genes, pathways, and regulatory networks within microbial communities.",
|
||||
"Offers an advantage over metagenomics by including both microbial species information and gene expression data. This dual focus provides a deeper understanding of microbial community changes from a transcriptional perspective and aids in the discovery of potential new genes."
|
||||
];
|
||||
|
||||
return (
|
||||
<AdvantagesLayout
|
||||
title="Advantages of Metatranscriptome Sequencing"
|
||||
advantageItems={advantageItems}
|
||||
/>
|
||||
);
|
||||
};
|
||||
|
||||
export default MetatranscriptomicsAdvantages;
|
||||
@ -0,0 +1,23 @@
|
||||
// MetatranscriptomicsApplications.jsx
|
||||
import ApplicationsLayout from '../../../components/shared/ApplicationsLayout';
|
||||
|
||||
const MetatranscriptomicsApplications = () => {
|
||||
const applicationItems = [
|
||||
"Metatranscriptomics sequencing is widely used in microbial genomics to explore microbial diversity, functional potentials, and ecological interactions across environments from soil to human microbiomes.",
|
||||
"Environmental Monitoring- Used to assess microbial community dynamics and functional potential in diverse environments like soil, oceans, and freshwater. It helps track microbial contributions to ecosystem processes and environmental health.",
|
||||
"Biogeochemical Cycling- Investigates the roles of microorganisms in nutrient cycling processes, such as carbon, nitrogen, and sulfur cycles, providing insights into how microbes influence these critical ecological functions.",
|
||||
"Disease Research- Explores the microbial contributions to human health and disease by studying the microbial communities in the human gut, oral cavity, or other body sites, revealing potential biomarkers and therapeutic targets.",
|
||||
"Biotechnology and Industry- Facilitates the discovery of novel enzymes and metabolic pathways from microbial communities, which can be applied in biotechnology, agriculture, and industrial processes.",
|
||||
"Microbial Interactions- Examines interactions between different microorganisms within complex communities, helping to understand symbiotic relationships, competition, and microbial networks.",
|
||||
"Pathogen Detection- Aids in identifying and characterizing pathogenic microbes and their gene expression profiles during infections, enhancing our ability to monitor and manage microbial diseases."
|
||||
];
|
||||
|
||||
return (
|
||||
<ApplicationsLayout
|
||||
title="Applications of Metatranscriptome Sequencing"
|
||||
applicationItems={applicationItems}
|
||||
/>
|
||||
);
|
||||
};
|
||||
|
||||
export default MetatranscriptomicsApplications;
|
||||
@ -0,0 +1,22 @@
|
||||
// app/rna-sequencing/metatranscriptomics-sequencing/components/MetatranscriptomicsIntroduction.jsx
|
||||
import IntroductionLayout from '../../../components/shared/IntroductionLayout';
|
||||
|
||||
const MetatranscriptomicsIntroduction = () => {
|
||||
const contentItems = [
|
||||
"Metatranscriptomics sequencing examines RNA transcripts from entire microbial communities within their natural environments during a specific time frame. This approach helps to infer the functional profile of a community under specific conditions, which are usually dependent on the status of the host.",
|
||||
"The process starts with the extraction of total RNA from a mixed microbial sample, without the need for bacterial isolation. Ribosomal RNA (rRNA) is then removed to enrich for mRNA and other non-ribosomal RNAs. The RNA is subsequently processed, prepared into libraries, and sequenced.",
|
||||
"The bioinformatics workflow includes preprocessing the raw sequencing reads, performing quality control, and normalizing the data. The reads are mapped to reference genomes or databases to quantify gene expression levels. Functional annotation and pathway analysis are then carried out to understand the roles of various transcripts and their impact on microbial community functions.",
|
||||
"Metatranscriptomics is applicable to a wide range of environmental samples, such as soil, oceans, and the human gut, facilitating studies on microbial diversity, disease mechanisms, nutrient cycling, and environmental adaptation."
|
||||
];
|
||||
|
||||
return (
|
||||
<IntroductionLayout
|
||||
title="Introduction and Workflow"
|
||||
contentItems={contentItems}
|
||||
badgeSubtext="Brochure Metatranscriptomics"
|
||||
useParagraphs={true}
|
||||
/>
|
||||
);
|
||||
};
|
||||
|
||||
export default MetatranscriptomicsIntroduction;
|
||||
@ -0,0 +1,57 @@
|
||||
// MetatranscriptomicsSpecifications.jsx
|
||||
import Link from 'next/link';
|
||||
import SpecificationsLayout from '../../../components/shared/SpecificationsLayout';
|
||||
|
||||
const MetatranscriptomicsSpecifications = () => {
|
||||
const specificationItems = [
|
||||
{
|
||||
icon: "/images/homepage-2/NGS-Icons-45.svg",
|
||||
title: "Sample Requirement",
|
||||
renderContent: () => (
|
||||
<div>
|
||||
<div className="mb-4">
|
||||
<p className="text-gray-600">Total RNA samples, isolated from various environmental samples such as soil, water, sediments, roots, leaves, animal gut/intestine etc,.</p>
|
||||
</div>
|
||||
<div className="mt-4 text-sm">
|
||||
<strong>
|
||||
Please refer to{' '}
|
||||
<Link href="/sample-submission-guidelines" className="text-teal-600 underline hover:text-teal-700">
|
||||
sample submission guidelines
|
||||
</Link>
|
||||
{' '}or{' '}
|
||||
<Link href="/contact" className="text-teal-600 underline hover:text-teal-700">
|
||||
Contact Us!
|
||||
</Link>
|
||||
</strong>
|
||||
</div>
|
||||
</div>
|
||||
)
|
||||
},
|
||||
{
|
||||
icon: "/images/homepage-1/service/Advantages-NGS-Icons-20.svg",
|
||||
title: "Sequencing Platform",
|
||||
content: "Illumina NovaSeq 6000/ NovaSeq X"
|
||||
},
|
||||
{
|
||||
icon: "/images/service/social-support.png",
|
||||
title: "Deliverables",
|
||||
renderContent: () => (
|
||||
<ul className="list-disc pl-5 space-y-2 text-gray-600 text-start">
|
||||
<li>The original sequencing data</li>
|
||||
<li>Experimental results</li>
|
||||
<li>Bioinformatics and Data analysis report</li>
|
||||
<li>Details of Metatranscriptome Sequencing (customizable)</li>
|
||||
</ul>
|
||||
)
|
||||
}
|
||||
];
|
||||
|
||||
return (
|
||||
<SpecificationsLayout
|
||||
title="Service Specifications"
|
||||
specificationItems={specificationItems}
|
||||
/>
|
||||
);
|
||||
};
|
||||
|
||||
export default MetatranscriptomicsSpecifications;
|
||||
27
app/rna-sequencing/metatranscriptomics-sequencing/page.js
Normal file
27
app/rna-sequencing/metatranscriptomics-sequencing/page.js
Normal file
@ -0,0 +1,27 @@
|
||||
import TitleBar from '../../components/shared/TitleBar';
|
||||
import MetatranscriptomicsIntroduction from './components/MetatranscriptomicsIntroduction';
|
||||
import MetatranscriptomicsAdvantages from './components/MetatranscriptomicsAdvantages';
|
||||
import MetatranscriptomicsApplications from './components/MetatranscriptomicsApplications';
|
||||
import MetatranscriptomicsSpecifications from './components/MetatranscriptomicsSpecifications';
|
||||
import PageLayout from '../../components/Layout/PageLayout';
|
||||
|
||||
export default function MetatranscriptomicsSequencingPage() {
|
||||
const breadcrumbs = [
|
||||
{ label: 'Home', href: '/', current: false },
|
||||
{ label: 'RNA Sequencing', href: '/rna-sequencing', current: false },
|
||||
{ label: 'Metatranscriptomics Sequencing', href: '#', current: true }
|
||||
];
|
||||
|
||||
return (
|
||||
<PageLayout fixedHeader={true}>
|
||||
<TitleBar
|
||||
title="Metatranscriptomics Sequencing"
|
||||
breadcrumbs={breadcrumbs}
|
||||
/>
|
||||
<MetatranscriptomicsIntroduction />
|
||||
<MetatranscriptomicsAdvantages />
|
||||
<MetatranscriptomicsApplications />
|
||||
<MetatranscriptomicsSpecifications />
|
||||
</PageLayout>
|
||||
);
|
||||
}
|
||||
@ -0,0 +1,20 @@
|
||||
// MRNAAdvantages.jsx
|
||||
import AdvantagesLayout from '../../../components/shared/AdvantagesLayout';
|
||||
|
||||
const MRNAAdvantages = () => {
|
||||
const advantageItems = [
|
||||
"mRNA-Seq provides a targeted analysis of gene expression specific to protein-coding regions, making it ideal for studies focused on gene regulation, protein synthesis, and disease-related expression changes.",
|
||||
"mRNA-Seq focuses on mRNA enrichment, which minimizes the impact of non-coding RNAs and enhances sensitivity for detecting gene expression changes.",
|
||||
"Focusing solely on coding regions, mRNA-Seq does not require rRNA-specific probes (Costly) for ribosomal RNA removal and requires comparatively lesser data, making it a more cost-effective alternative to Whole Transcriptome Sequencing.",
|
||||
"With a more targeted dataset, the bioinformatics analysis of mRNA-Seq is often simpler and faster, focusing on coding genes without the complexity introduced by non-coding RNAs."
|
||||
];
|
||||
|
||||
return (
|
||||
<AdvantagesLayout
|
||||
title="Advantages of mRNA Sequencing (mRNA-Seq) over Whole Transcriptome Sequencing"
|
||||
advantageItems={advantageItems}
|
||||
/>
|
||||
);
|
||||
};
|
||||
|
||||
export default MRNAAdvantages;
|
||||
@ -0,0 +1,23 @@
|
||||
// MRNAApplications.jsx
|
||||
import ApplicationsLayout from '../../../components/shared/ApplicationsLayout';
|
||||
|
||||
const MRNAApplications = () => {
|
||||
const applicationItems = [
|
||||
"mRNA sequencing is widely utilized across plants, animals, microbes, and humans.",
|
||||
"Developmental Biology- Studies gene expression patterns during different stages of development, helping to unravel the mechanisms of cell differentiation and tissue formation.",
|
||||
"Disease Mechanisms- Uncovers mRNA expression profiles associated with various diseases, including genetic disorders and autoimmune conditions, to identify potential diagnostic markers and therapeutic targets.",
|
||||
"Drug Discovery- Analyzes mRNA expression changes in response to drug treatments, providing insights into drug mechanisms of action and helping to identify potential drug candidates.",
|
||||
"Functional Genomics- Provides a comprehensive view of gene expression across different conditions, facilitating the study of gene functions and regulatory networks.",
|
||||
"Comparative Genomics- Compares mRNA expression profiles across different species or environmental conditions to understand evolutionary differences and functional conservation.",
|
||||
"Toxicology- Assesses changes in gene expression in response to toxic substances, aiding in the identification of biomarkers for toxicity and understanding the impact of chemicals on biological systems."
|
||||
];
|
||||
|
||||
return (
|
||||
<ApplicationsLayout
|
||||
title="Applications of mRNA Sequencing"
|
||||
applicationItems={applicationItems}
|
||||
/>
|
||||
);
|
||||
};
|
||||
|
||||
export default MRNAApplications;
|
||||
@ -0,0 +1,23 @@
|
||||
// app/rna-sequencing/mrna-sequencing/components/MRNAIntroduction.jsx
|
||||
import IntroductionLayout from '../../../components/shared/IntroductionLayout';
|
||||
|
||||
const MRNAIntroduction = () => {
|
||||
const contentItems = [
|
||||
"Messenger RNA-Seq (mRNA-Seq) is a focused RNA-Seq method that targets polyadenylated (poly-A) transcripts, which comprise only about 1-2% of the transcriptome, mainly representing coding genes. By enriching these poly-A-tailed RNAs, mRNA-Seq offers a precise snapshot of gene expression, capturing the complete range of mRNA transcripts within a sample and enabling detailed analysis of gene activity across various conditions.",
|
||||
"mRNA-seq workflow typically includes RNA extraction, enrichment or depletion of mRNA, library preparation, and high-throughput sequencing. This workflow ensures precise and efficient capture of mRNA transcripts for thorough downstream analysis.",
|
||||
"Bioinformatics analysis for mRNA-seq involves several key steps: data preprocessing, alignment to a reference genome, quantifying gene expression levels, and advanced downstream analyses such as differential expression, pathway enrichment, and gene ontology analysis.",
|
||||
"Widely applicable across diverse research fields, including human, animal, and plant studies, providing profound insights into genetic landscapes and contributing to our understanding of gene function and regulation."
|
||||
];
|
||||
|
||||
return (
|
||||
<IntroductionLayout
|
||||
title="Introduction and Workflow"
|
||||
contentItems={contentItems}
|
||||
imageUrl="https://stock.adobe.com/in/images/messenger-rna-or-mrna-strand-3d-rendering-illustration-with-copy-space-genetics-science-medical-research-genome-replication-concepts/404350568"
|
||||
imageAlt="mRNA Sequencing Workflow"
|
||||
useParagraphs={true}
|
||||
/>
|
||||
);
|
||||
};
|
||||
|
||||
export default MRNAIntroduction;
|
||||
@ -0,0 +1,58 @@
|
||||
// MRNASpecifications.jsx
|
||||
import Link from 'next/link';
|
||||
import SpecificationsLayout from '../../../components/shared/SpecificationsLayout';
|
||||
|
||||
const MRNASpecifications = () => {
|
||||
const specificationItems = [
|
||||
{
|
||||
icon: "/images/homepage-2/NGS-Icons-45.svg",
|
||||
title: "Sample Requirement",
|
||||
renderContent: () => (
|
||||
<div>
|
||||
<div className="mb-4">
|
||||
<p className="text-gray-600">Total RNA ≥ 2 μg, Minimum Quantity: 500 ng, Concentration≥ 50 ng/µL</p>
|
||||
<p className="text-gray-600">Cells, Tissue and other samples</p>
|
||||
</div>
|
||||
<div className="mt-4 text-sm">
|
||||
<strong>
|
||||
Please refer to{' '}
|
||||
<Link href="/sample-submission-guidelines" className="text-teal-600 underline hover:text-teal-700">
|
||||
sample submission guidelines
|
||||
</Link>
|
||||
{' '}or{' '}
|
||||
<Link href="/contact" className="text-teal-600 underline hover:text-teal-700">
|
||||
Contact Us!
|
||||
</Link>
|
||||
</strong>
|
||||
</div>
|
||||
</div>
|
||||
)
|
||||
},
|
||||
{
|
||||
icon: "/images/homepage-1/service/Advantages-NGS-Icons-20.svg",
|
||||
title: "Sequencing Platform",
|
||||
content: "Illumina NovaSeq 6000/ NovaSeq X"
|
||||
},
|
||||
{
|
||||
icon: "/images/service/social-support.png",
|
||||
title: "Deliverables",
|
||||
renderContent: () => (
|
||||
<ul className="list-disc pl-5 space-y-2 text-gray-600 text-start">
|
||||
<li>The original sequencing data</li>
|
||||
<li>Experimental results</li>
|
||||
<li>Bioinformatics and Data analysis report</li>
|
||||
<li>Details of mRNA Sequencing (customizable)</li>
|
||||
</ul>
|
||||
)
|
||||
}
|
||||
];
|
||||
|
||||
return (
|
||||
<SpecificationsLayout
|
||||
title="Service Specifications"
|
||||
specificationItems={specificationItems}
|
||||
/>
|
||||
);
|
||||
};
|
||||
|
||||
export default MRNASpecifications;
|
||||
28
app/rna-sequencing/mrna-sequencing/page.js
Normal file
28
app/rna-sequencing/mrna-sequencing/page.js
Normal file
@ -0,0 +1,28 @@
|
||||
// app/rna-sequencing/mrna-sequencing/page.js
|
||||
import TitleBar from '../../components/shared/TitleBar';
|
||||
import MRNAIntroduction from './components/MRNAIntroduction';
|
||||
import MRNAAdvantages from './components/MRNAAdvantages';
|
||||
import MRNAApplications from './components/MRNAApplications';
|
||||
import MRNASpecifications from './components/MRNASpecifications';
|
||||
import PageLayout from '../../components/Layout/PageLayout';
|
||||
|
||||
export default function MRNASequencingPage() {
|
||||
const breadcrumbs = [
|
||||
{ label: 'Home', href: '/', current: false },
|
||||
{ label: 'RNA Sequencing', href: '/rna-sequencing', current: false },
|
||||
{ label: 'mRNA Sequencing', href: '#', current: true }
|
||||
];
|
||||
|
||||
return (
|
||||
<PageLayout fixedHeader={true}>
|
||||
<TitleBar
|
||||
title="mRNA Sequencing"
|
||||
breadcrumbs={breadcrumbs}
|
||||
/>
|
||||
<MRNAIntroduction />
|
||||
<MRNAAdvantages />
|
||||
<MRNAApplications />
|
||||
<MRNASpecifications />
|
||||
</PageLayout>
|
||||
);
|
||||
}
|
||||
@ -0,0 +1,20 @@
|
||||
// SingleCellAdvantages.jsx (RNA version)
|
||||
import AdvantagesLayout from '../../../components/shared/AdvantagesLayout';
|
||||
|
||||
const SingleCellAdvantages = () => {
|
||||
const advantageItems = [
|
||||
"Unlike traditional bulk RNA sequencing, which averages gene expression across many cells and can hide unique cellular differences, single-cell RNA sequencing examines each cell individually. This reveals variations in gene expression and provides detailed insights into cellular functions, development, and disease mechanisms that are often lost in population-level data.",
|
||||
"Enables the discovery and characterization of rare or previously unrecognized cell populations, essential for understanding complex biological systems and disease mechanisms.",
|
||||
"Delivers high-resolution data on gene expression patterns, allowing for precise mapping of gene regulatory networks and cellular states, and uncovering intricate details of cellular interactions.",
|
||||
"Supports the identification of biomarkers and therapeutic targets by offering a detailed snapshot of gene activity specific to individual cell types or states, facilitating more targeted and effective research and treatments."
|
||||
];
|
||||
|
||||
return (
|
||||
<AdvantagesLayout
|
||||
title="Advantages of Single Cell RNA Sequencing"
|
||||
advantageItems={advantageItems}
|
||||
/>
|
||||
);
|
||||
};
|
||||
|
||||
export default SingleCellAdvantages;
|
||||
@ -0,0 +1,21 @@
|
||||
// SingleCellApplications.jsx (RNA version)
|
||||
import ApplicationsLayout from '../../../components/shared/ApplicationsLayout';
|
||||
|
||||
const SingleCellApplications = () => {
|
||||
const applicationItems = [
|
||||
"Single Cell RNA sequencing is widely utilized across plants, animals, and humans.",
|
||||
"Cellular Heterogeneity- Enables the identification and characterization of distinct cell types within complex tissues, providing insights into the diversity and functions of individual cells in health and disease.",
|
||||
"Developmental Biology- Reveals how gene expression changes during development and differentiation, helping to understand the mechanisms driving cell fate decisions and tissue formation.",
|
||||
"Immunology- Provides detailed profiles of immune cell types and states, aiding in the study of immune responses, autoimmune diseases, and vaccine development by uncovering the dynamics of individual immune cells.",
|
||||
"Cancer Research- Helps in identifying rare tumor cell populations, understanding tumor heterogeneity, and discovering novel biomarkers or therapeutic targets specific to different cancer subtypes."
|
||||
];
|
||||
|
||||
return (
|
||||
<ApplicationsLayout
|
||||
title="Applications of Single Cell RNA Sequencing"
|
||||
applicationItems={applicationItems}
|
||||
/>
|
||||
);
|
||||
};
|
||||
|
||||
export default SingleCellApplications;
|
||||
@ -0,0 +1,22 @@
|
||||
// app/rna-sequencing/single-cell-rna-sequencing/components/SingleCellIntroduction.jsx
|
||||
import IntroductionLayout from '../../../components/shared/IntroductionLayout';
|
||||
|
||||
const SingleCellIntroduction = () => {
|
||||
const contentItems = [
|
||||
"Single Cell RNA Sequencing (scRNA-seq) analyzes gene expression at the resolution of individual cells, allowing for the exploration of cellular transcriptomic heterogeneity and the discovery of rare cell types within complex tissues.",
|
||||
"The workflow involves isolating individual cells from a sample, often using techniques like fluorescence-activated cell sorting (FACS), followed by RNA extraction, library preparation and (or) all the mRNA molecules in each cell are specifically barcoded either by capturing individual cells in a gel-emulsions (Gem; 10X) or Split-pool approach.",
|
||||
"The bioinformatics pipeline includes data preprocessing, which involves quality control and normalization of the sequencing reads. The reads are then aligned to a reference genome or transcriptome to identify gene expression levels in each cell. Subsequent analysis involves clustering of cells based on expression profiles, differential gene expression analysis, and the identification of cell types and states.",
|
||||
"Single-cell high-resolution transcriptome profiling offers valuable insights into gene regulatory networks and unveils diverse gene expression patterns within individual cells across cell cultures, tissues, and organs."
|
||||
];
|
||||
|
||||
return (
|
||||
<IntroductionLayout
|
||||
title="Introduction and Workflow"
|
||||
contentItems={contentItems}
|
||||
badgeSubtext="Workflow from Single Cell RNA Sequencing"
|
||||
useParagraphs={true}
|
||||
/>
|
||||
);
|
||||
};
|
||||
|
||||
export default SingleCellIntroduction;
|
||||
@ -0,0 +1,57 @@
|
||||
// app/rna-sequencing/single-cell-rna-sequencing/components/SingleCellSpecifications.jsx
|
||||
import Link from 'next/link';
|
||||
import SpecificationsLayout from '../../../components/shared/SpecificationsLayout';
|
||||
|
||||
const SingleCellSpecifications = () => {
|
||||
const specificationItems = [
|
||||
{
|
||||
icon: "/images/homepage-2/NGS-Icons-45.svg",
|
||||
title: "Sample Requirement",
|
||||
renderContent: () => (
|
||||
<div>
|
||||
<div className="mb-4">
|
||||
<p className="text-gray-600">Fresh Viable Samples - Individual Cells for Animal and Protoplasm for Plants</p>
|
||||
</div>
|
||||
<div className="mt-4 text-sm">
|
||||
<strong>
|
||||
Please refer to{' '}
|
||||
<Link href="/sample-submission-guideline" className="text-teal-600 underline hover:text-teal-700">
|
||||
sample submission guidelines
|
||||
</Link>
|
||||
{' '}or{' '}
|
||||
<Link href="/contact-us" className="text-teal-600 underline hover:text-teal-700">
|
||||
Contact Us!
|
||||
</Link>
|
||||
</strong>
|
||||
</div>
|
||||
</div>
|
||||
)
|
||||
},
|
||||
{
|
||||
icon: "/images/homepage-1/service/Advantages-NGS-Icons-20.svg",
|
||||
title: "Sequencing Platform",
|
||||
content: "Illumina NovaSeq 6000/ NovaSeq X"
|
||||
},
|
||||
{
|
||||
icon: "/images/service/social-support.png",
|
||||
title: "Deliverables",
|
||||
renderContent: () => (
|
||||
<ul className="list-disc pl-5 space-y-2 text-gray-600 text-start">
|
||||
<li>The original sequencing data</li>
|
||||
<li>Experimental results</li>
|
||||
<li>Bioinformatics and Data Analysis Report</li>
|
||||
<li>Details of Single Cell RNA Sequencing (customizable)</li>
|
||||
</ul>
|
||||
)
|
||||
}
|
||||
];
|
||||
|
||||
return (
|
||||
<SpecificationsLayout
|
||||
title="Service Specifications"
|
||||
specificationItems={specificationItems}
|
||||
/>
|
||||
);
|
||||
};
|
||||
|
||||
export default SingleCellSpecifications;
|
||||
27
app/rna-sequencing/single-cell-rna-sequencing/page.js
Normal file
27
app/rna-sequencing/single-cell-rna-sequencing/page.js
Normal file
@ -0,0 +1,27 @@
|
||||
import TitleBar from '../../components/shared/TitleBar';
|
||||
import SingleCellIntroduction from './components/SingleCellIntroduction';
|
||||
import SingleCellAdvantages from './components/SingleCellAdvantages';
|
||||
import SingleCellApplications from './components/SingleCellApplications';
|
||||
import SingleCellSpecifications from './components/SingleCellSpecifications';
|
||||
import PageLayout from '../../components/Layout/PageLayout';
|
||||
|
||||
export default function SingleCellRNASequencingPage() {
|
||||
const breadcrumbs = [
|
||||
{ label: 'Home', href: '/', current: false },
|
||||
{ label: 'RNA Sequencing', href: '/rna-sequencing', current: false },
|
||||
{ label: 'Single Cell RNA Sequencing', href: '#', current: true }
|
||||
];
|
||||
|
||||
return (
|
||||
<PageLayout>
|
||||
<TitleBar
|
||||
title="Single Cell RNA Sequencing"
|
||||
breadcrumbs={breadcrumbs}
|
||||
/>
|
||||
<SingleCellIntroduction />
|
||||
<SingleCellAdvantages />
|
||||
<SingleCellApplications />
|
||||
<SingleCellSpecifications />
|
||||
</PageLayout>
|
||||
);
|
||||
}
|
||||
@ -0,0 +1,20 @@
|
||||
// SRNAAdvantages.jsx
|
||||
import AdvantagesLayout from '../../../components/shared/AdvantagesLayout';
|
||||
|
||||
const SRNAAdvantages = () => {
|
||||
const advantageItems = [
|
||||
"Provides a comprehensive view of the small RNA landscape by capturing a broad range of small RNA species, including miRNAs, siRNAs, snoRNAs, piRNAs, and tasiRNAs.",
|
||||
"Facilitates comparative studies by allowing the comparison of small RNA expression profiles across different conditions or species, supporting investigations into developmental, environmental, and disease-related changes.",
|
||||
"Identifies previously unmapped small RNAs and isoforms, as well as novel biomarkers, expanding the scope of genetic research and potential clinical applications.",
|
||||
"Enhances understanding of how post-transcriptional regulation affects phenotypes and offers detailed functional insights into small RNA-target interactions and their roles in various cellular pathways, improving the understanding of gene regulation and function."
|
||||
];
|
||||
|
||||
return (
|
||||
<AdvantagesLayout
|
||||
title="Advantages of Small RNA (sRNA) sequencing (miRNA)"
|
||||
advantageItems={advantageItems}
|
||||
/>
|
||||
);
|
||||
};
|
||||
|
||||
export default SRNAAdvantages;
|
||||
@ -0,0 +1,24 @@
|
||||
// SRNAApplications.jsx
|
||||
import ApplicationsLayout from '../../../components/shared/ApplicationsLayout';
|
||||
|
||||
const SRNAApplications = () => {
|
||||
const applicationItems = [
|
||||
"sRNA sequencing is widely utilized across plants, animals, microbes, and humans.",
|
||||
"Cancer Research- Identifies novel small RNAs and biomarkers, revealing their roles in tumor development and progression, and aiding in the discovery of potential therapeutic targets.",
|
||||
"Developmental Biology- Examines small RNA expression during development to understand gene regulation, cellular differentiation, and developmental disorders.",
|
||||
"Genetic Studies- Analyzes allele-specific expression and variations in small RNAs to gain insights into genetic regulation and variability.",
|
||||
"Microbial Genomics- Investigates small RNA profiles in bacteria, aiding in the understanding of gene regulation, host-pathogen interactions, and antibiotic resistance.",
|
||||
"Neuroscience- Explores small RNA involvement in brain function and neurodevelopmental disorders, offering insights into neural regulation and disease mechanisms.",
|
||||
"Immunology- Studies small RNA expression in immune cells to understand immune responses, autoimmune diseases, and immune system regulation.",
|
||||
"Environmental and Ecological Research- Analyzes the impact of environmental changes on small RNA profiles in plants, animals, and microbes, supporting studies on adaptation and stress responses."
|
||||
];
|
||||
|
||||
return (
|
||||
<ApplicationsLayout
|
||||
title="Applications of Small RNA (sRNA) sequencing"
|
||||
applicationItems={applicationItems}
|
||||
/>
|
||||
);
|
||||
};
|
||||
|
||||
export default SRNAApplications;
|
||||
@ -0,0 +1,33 @@
|
||||
// app/rna-sequencing/small-rna-sequencing/components/SRNABioinformatics.jsx
|
||||
|
||||
const SRNABioinformatics = () => {
|
||||
return (
|
||||
<section className="py-8 lg:py-12 bg-gray-50">
|
||||
<div className="container mx-auto max-w-none px-4 lg:px-6">
|
||||
<h2 className="text-2xl lg:text-3xl text-gray-700 text-left pb-2 mb-6 lg:mb-6">
|
||||
Bioinformatics Pipeline
|
||||
</h2>
|
||||
|
||||
{/* Pipeline Image */}
|
||||
<div className="bg-white rounded-xl shadow-lg p-6 lg:p-8">
|
||||
<div className="flex justify-center">
|
||||
<img
|
||||
src="/images/srna-bioinformatics-pipeline.jpg"
|
||||
alt="Small RNA Sequencing Bioinformatics Pipeline Workflow"
|
||||
className="max-w-full h-auto rounded-lg"
|
||||
/>
|
||||
</div>
|
||||
|
||||
{/* Pipeline Description */}
|
||||
<div className="mt-6 text-center">
|
||||
<p className="text-gray-600 text-sm lg:text-base">
|
||||
Small RNA sequencing bioinformatics pipeline for miRNA and small RNA analysis and expression profiling
|
||||
</p>
|
||||
</div>
|
||||
</div>
|
||||
</div>
|
||||
</section>
|
||||
);
|
||||
};
|
||||
|
||||
export default SRNABioinformatics;
|
||||
@ -0,0 +1,22 @@
|
||||
// app/rna-sequencing/small-rna-sequencing/components/SRNAIntroduction.jsx
|
||||
import IntroductionLayout from '../../../components/shared/IntroductionLayout';
|
||||
|
||||
const SRNAIntroduction = () => {
|
||||
const contentItems = [
|
||||
"Small RNA sequencing (sRNA-seq) is designed to identify and analyze small non-coding RNAs (ncRNAs) that are under 200 nucleotides in length. These small RNAs, including microRNAs (miRNAs), small interfering RNAs (siRNAs), small nucleolar RNAs (snoRNAs), PIWI-interacting RNAs (piRNAs), and trans-acting siRNAs (tasiRNAs), play crucial roles in gene silencing and post-transcriptional regulation.",
|
||||
"The sRNA-seq workflow involves isolating miRNAs from samples, followed by preparing libraries with specific adapters and sequencing them using high-throughput platforms.",
|
||||
"The bioinformatics pipeline for sRNA-seq encompasses data preprocessing, alignment with reference genomes, and identification of small RNA species. This analysis includes differential expression studies, characterization of novel small RNAs, and pathway analysis to gain functional insights into sRNA-target interactions.",
|
||||
"Facilitates the discovery of novel small RNAs and offers detailed insights into their expression and functions. It links post-transcriptional regulation to phenotypic outcomes, aiding in the understanding of disease mechanisms and the identification of new biomarkers."
|
||||
];
|
||||
|
||||
return (
|
||||
<IntroductionLayout
|
||||
title="Introduction and Workflow"
|
||||
contentItems={contentItems}
|
||||
badgeSubtext="from brochure Small RNA sequencing"
|
||||
useParagraphs={true}
|
||||
/>
|
||||
);
|
||||
};
|
||||
|
||||
export default SRNAIntroduction;
|
||||
@ -0,0 +1,57 @@
|
||||
// SRNASpecifications.jsx
|
||||
import Link from 'next/link';
|
||||
import SpecificationsLayout from '../../../components/shared/SpecificationsLayout';
|
||||
|
||||
const SRNASpecifications = () => {
|
||||
const specificationItems = [
|
||||
{
|
||||
icon: "/images/homepage-2/NGS-Icons-45.svg",
|
||||
title: "Sample Requirement",
|
||||
renderContent: () => (
|
||||
<div>
|
||||
<div className="mb-4">
|
||||
<p className="text-gray-600">Total RNA ≥ 1 μg, Cells≥ 2×10<sup>6</sup>, or Tissue ≥ 500 mg.</p>
|
||||
</div>
|
||||
<div className="mt-4 text-sm">
|
||||
<strong>
|
||||
Please refer to{' '}
|
||||
<Link href="/sample-submission-guidelines" className="text-teal-600 underline hover:text-teal-700">
|
||||
sample submission guidelines
|
||||
</Link>
|
||||
{' '}or{' '}
|
||||
<Link href="/contact" className="text-teal-600 underline hover:text-teal-700">
|
||||
Contact Us!
|
||||
</Link>
|
||||
</strong>
|
||||
</div>
|
||||
</div>
|
||||
)
|
||||
},
|
||||
{
|
||||
icon: "/images/homepage-1/service/Advantages-NGS-Icons-20.svg",
|
||||
title: "Sequencing Platform",
|
||||
content: "Illumina MiSeq, Nextseq 550"
|
||||
},
|
||||
{
|
||||
icon: "/images/service/social-support.png",
|
||||
title: "Deliverables",
|
||||
renderContent: () => (
|
||||
<ul className="list-disc pl-5 space-y-2 text-gray-600 text-start">
|
||||
<li>The original sequencing data</li>
|
||||
<li>Experimental results</li>
|
||||
<li>Bioinformatics and Data analysis report</li>
|
||||
<li>Details in Small RNA Sequencing for your writing (customization)</li>
|
||||
</ul>
|
||||
)
|
||||
}
|
||||
];
|
||||
|
||||
return (
|
||||
<SpecificationsLayout
|
||||
title="Service Specifications"
|
||||
specificationItems={specificationItems}
|
||||
/>
|
||||
);
|
||||
};
|
||||
|
||||
export default SRNASpecifications;
|
||||
30
app/rna-sequencing/small-rna-sequencing/page.js
Normal file
30
app/rna-sequencing/small-rna-sequencing/page.js
Normal file
@ -0,0 +1,30 @@
|
||||
// app/rna-sequencing/small-rna-sequencing/page.js
|
||||
import TitleBar from '../../components/shared/TitleBar';
|
||||
import SRNAIntroduction from './components/SRNAIntroduction';
|
||||
import SRNAAdvantages from './components/SRNAAdvantages';
|
||||
import SRNABioinformatics from './components/SRNABioinformatics';
|
||||
import SRNAApplications from './components/SRNAApplications';
|
||||
import SRNASpecifications from './components/SRNASpecifications';
|
||||
import PageLayout from '../../components/Layout/PageLayout';
|
||||
|
||||
export default function SmallRNASequencingPage() {
|
||||
const breadcrumbs = [
|
||||
{ label: 'Home', href: '/', current: false },
|
||||
{ label: 'RNA Sequencing', href: '/rna-sequencing', current: false },
|
||||
{ label: 'Small RNA (sRNA) sequencing', href: '#', current: true }
|
||||
];
|
||||
|
||||
return (
|
||||
<PageLayout fixedHeader={true}>
|
||||
<TitleBar
|
||||
title="Small RNA (sRNA) sequencing"
|
||||
breadcrumbs={breadcrumbs}
|
||||
/>
|
||||
<SRNAIntroduction />
|
||||
<SRNAAdvantages />
|
||||
<SRNABioinformatics />
|
||||
<SRNAApplications />
|
||||
<SRNASpecifications />
|
||||
</PageLayout>
|
||||
);
|
||||
}
|
||||
@ -0,0 +1,22 @@
|
||||
// WTSAdvantages.jsx
|
||||
import AdvantagesLayout from '../../../components/shared/AdvantagesLayout';
|
||||
|
||||
const WTSAdvantages = () => {
|
||||
const advantageItems = [
|
||||
"Enables the identification of novel transcripts, alternative splicing events, post-transcriptional modifications, and gene fusions without prior knowledge, making it ideal for exploratory research.",
|
||||
"Analyzing the entire transcriptome helps gain a deeper understanding of gene regulation, cellular responses, and pathways, leading to a more comprehensive view of biological processes.",
|
||||
"Enables allele-specific gene expression analysis, offering valuable insights into gene regulation and genetic variability.",
|
||||
"Facilitates higher detection rates of gene fusions, indels, SNPs, and other genetic variations, offering greater reliability in research outcomes.",
|
||||
"Widely used in fields such as cancer research and developmental biology, WTS uncovers complex molecular mechanisms and aids in the discovery of potential biomarkers.",
|
||||
"Provides valuable insights into bacterial gene expression, helping to unravel the mysteries of gene regulation, host-pathogen interactions, antibiotic resistance, and the underlying causes of various diseases."
|
||||
];
|
||||
|
||||
return (
|
||||
<AdvantagesLayout
|
||||
title="Advantages of Whole Transcriptome Sequencing (WTS)"
|
||||
advantageItems={advantageItems}
|
||||
/>
|
||||
);
|
||||
};
|
||||
|
||||
export default WTSAdvantages;
|
||||
@ -0,0 +1,24 @@
|
||||
// app/rna-sequencing/whole-transcriptome-sequencing/components/WTSApplications.jsx
|
||||
import ApplicationsLayout from '../../../components/shared/ApplicationsLayout';
|
||||
|
||||
const WTSApplications = () => {
|
||||
const applicationItems = [
|
||||
"Developmental Biology- Explores gene expression changes during development, providing insights into cellular differentiation and organismal growth.",
|
||||
"Genetic Research- Enables allele-specific expression analysis, enhancing the understanding of genetic variability and gene regulation.",
|
||||
"Microbial Studies- Examines microbial gene expression, host-pathogen interactions, and antibiotic resistance, providing insights into microbial ecology and disease prevention.",
|
||||
"Human Health- Identifies gene fusions, mutations, and expression profiles in cancer, neurological disorders, and other diseases, aiding in the discovery of biomarkers and therapeutic targets.",
|
||||
"Immunology- Analyzes immune response pathways and gene expression in various immune cell types, contributing to improving our understanding of immune system function and disorders.",
|
||||
"Environmental Biology- Assesses the impact of environmental changes on gene expression in plants, animals, and microbes, aiding in ecological and evolutionary studies.",
|
||||
"Plant Genomics- Investigates gene expression during growth, stress responses, and development, aiding in crop improvement and understanding plant-environment interactions.",
|
||||
"Animal Genomics- Explores gene regulation, development, and disease mechanisms across various animal species, contributing to veterinary research and comparative genomics."
|
||||
];
|
||||
|
||||
return (
|
||||
<ApplicationsLayout
|
||||
title="Applications of Whole Transcriptome Sequencing (WTS)"
|
||||
applicationItems={applicationItems}
|
||||
/>
|
||||
);
|
||||
};
|
||||
|
||||
export default WTSApplications;
|
||||
@ -0,0 +1,23 @@
|
||||
// app/rna-sequencing/whole-transcriptome-sequencing/components/WTSIntroduction.jsx
|
||||
import IntroductionLayout from '../../../components/shared/IntroductionLayout';
|
||||
|
||||
const WTSIntroduction = () => {
|
||||
const contentItems = [
|
||||
"Whole Transcriptome Sequencing (WTS) comprehensively captures and quantifies all RNA transcripts, both coding (mRNA) and long non-coding (rRNA, tRNA), providing an in-depth view of gene expression.",
|
||||
"This method combines a ribosomal RNA depletion strategy with Illumina NGS technology for efficient and precise results. The approach (1) reduces ribosomal contamination and increases the proportion of uniquely mapped reads, and (2) is particularly beneficial for species, such as bacteria, whose mRNA may lack a poly-A tail.",
|
||||
"The bioinformatics pipeline includes data preprocessing, genome mapping, transcript identification, and downstream analyses like differential expression analysis, lncRNA target gene analysis, GO enrichment analysis, and KEGG enrichment analysis.",
|
||||
"This technique is applicable to a wide range of species, including humans, mouse, rat, other animals, plants, and prokaryotes. It is compatible with various samples like; high and low-quality RNA samples, cell-free RNA, formalin-fixed paraffin-embedded (FFPE) tissues and RNA."
|
||||
];
|
||||
|
||||
return (
|
||||
<IntroductionLayout
|
||||
title="Introduction and Workflow"
|
||||
contentItems={contentItems}
|
||||
imageUrl="https://stock.adobe.com/in/images/messenger-rna-or-mrna-strand-3d-rendering-illustration-with-copy-space-genetics-science-medical-research-genome-replication-concepts/404350568"
|
||||
imageAlt="Whole Transcriptome Sequencing Workflow"
|
||||
useParagraphs={true}
|
||||
/>
|
||||
);
|
||||
};
|
||||
|
||||
export default WTSIntroduction;
|
||||
@ -0,0 +1,58 @@
|
||||
// WTSSpecifications.jsx
|
||||
import Link from 'next/link';
|
||||
import SpecificationsLayout from '../../../components/shared/SpecificationsLayout';
|
||||
|
||||
const WTSSpecifications = () => {
|
||||
const specificationItems = [
|
||||
{
|
||||
icon: "/images/homepage-2/NGS-Icons-45.svg",
|
||||
title: "Sample Requirement",
|
||||
renderContent: () => (
|
||||
<div>
|
||||
<div className="mb-4">
|
||||
<p className="text-gray-600">Total RNA ≥ 2 μg, Minimum Quantity: 500 ng, Concentration≥ 50 ng/µL</p>
|
||||
<p className="text-gray-600">Cells, Tissue and other samples</p>
|
||||
</div>
|
||||
<div className="mt-4 text-sm">
|
||||
<strong>
|
||||
Please refer to{' '}
|
||||
<Link href="/sample-submission-guidelines" className="text-teal-600 underline hover:text-teal-700">
|
||||
sample submission guidelines
|
||||
</Link>
|
||||
{' '}or{' '}
|
||||
<Link href="/contact" className="text-teal-600 underline hover:text-teal-700">
|
||||
Contact Us!
|
||||
</Link>
|
||||
</strong>
|
||||
</div>
|
||||
</div>
|
||||
)
|
||||
},
|
||||
{
|
||||
icon: "/images/homepage-1/service/Advantages-NGS-Icons-20.svg",
|
||||
title: "Sequencing Platform",
|
||||
content: "Illumina NovaSeq 6000/ NovaSeq X Plus"
|
||||
},
|
||||
{
|
||||
icon: "/images/service/social-support.png",
|
||||
title: "Deliverables",
|
||||
renderContent: () => (
|
||||
<ul className="list-disc pl-5 space-y-2 text-gray-600 text-start">
|
||||
<li>The original sequencing data</li>
|
||||
<li>Experimental results</li>
|
||||
<li>Bioinformatics and Data Analysis Report</li>
|
||||
<li>Details in Total RNA Sequencing for your writing (customization)</li>
|
||||
</ul>
|
||||
)
|
||||
}
|
||||
];
|
||||
|
||||
return (
|
||||
<SpecificationsLayout
|
||||
title="Service Specifications"
|
||||
specificationItems={specificationItems}
|
||||
/>
|
||||
);
|
||||
};
|
||||
|
||||
export default WTSSpecifications;
|
||||
28
app/rna-sequencing/whole-transcriptome-sequencing/page.js
Normal file
28
app/rna-sequencing/whole-transcriptome-sequencing/page.js
Normal file
@ -0,0 +1,28 @@
|
||||
// app/rna-sequencing/whole-transcriptome-sequencing/page.js
|
||||
import TitleBar from '../../components/shared/TitleBar';
|
||||
import WTSIntroduction from './components/WTSIntroduction';
|
||||
import WTSAdvantages from './components/WTSAdvantages';
|
||||
import WTSApplications from './components/WTSApplications';
|
||||
import WTSSpecifications from './components/WTSSpecifications';
|
||||
import PageLayout from '../../components/Layout/PageLayout';
|
||||
|
||||
export default function WholeTranscriptomeSequencingPage() {
|
||||
const breadcrumbs = [
|
||||
{ label: 'Home', href: '/', current: false },
|
||||
{ label: 'RNA Sequencing', href: '/rna-sequencing', current: false },
|
||||
{ label: 'Whole Transcriptome (Total RNA) Sequencing', href: '#', current: true }
|
||||
];
|
||||
|
||||
return (
|
||||
<PageLayout fixedHeader={true}>
|
||||
<TitleBar
|
||||
title="Whole Transcriptome (Total RNA) Sequencing"
|
||||
breadcrumbs={breadcrumbs}
|
||||
/>
|
||||
<WTSIntroduction />
|
||||
<WTSAdvantages />
|
||||
<WTSApplications />
|
||||
<WTSSpecifications />
|
||||
</PageLayout>
|
||||
);
|
||||
}
|
||||
Reference in New Issue
Block a user