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// DegradomeAdvantages.jsx
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import AdvantagesLayout from '../../../components/shared/AdvantagesLayout';
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const DegradomeAdvantages = () => {
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const advantageItems = [
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"Degradome sequencing provides a focused analysis of RNA degradation, specifically targeting cleavage sites mediated by miRNAs and ta-siRNAs, making it ideal for studying gene regulation and RNA stability.",
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"By targeting degradation products, degradome sequencing minimizes the impact of intact RNAs, enhancing sensitivity for detecting miRNA targets and cleavage events.",
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"Degradome sequencing focuses on RNA cleavage sites, eliminating the need for additional steps like ribosomal RNA removal, making it a more cost-effective alternative to broader transcriptome studies.",
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"Degradome sequencing enables the identification of novel regulatory elements, such as circRNAs and biomarkers, expanding its utility in understanding RNA regulatory networks."
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];
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return (
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<AdvantagesLayout
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title="Advantages of Degradome Sequencing"
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advantageItems={advantageItems}
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/>
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);
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};
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export default DegradomeAdvantages;
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// DegradomeApplications.jsx
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import ApplicationsLayout from '../../../components/shared/ApplicationsLayout';
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const DegradomeApplications = () => {
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const applicationItems = [
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"Gene Regulation Studies- Used to identify miRNA targets and cleavage sites, elucidating the regulatory networks that control gene expression and RNA turnover in different organisms.",
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"Developmental Biology- Explores RNA degradation patterns during developmental stages, uncovering the molecular mechanisms underlying cell differentiation and tissue formation.",
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"Disease Research- Investigates RNA cleavage profiles associated with diseases such as cancer and neurodegenerative disorders, helping to identify potential biomarkers and therapeutic targets.",
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"Functional Genomics- Provides a detailed view of RNA decay dynamics, enabling the study of gene function and the roles of regulatory RNAs in cellular processes.",
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"Comparative Genomics- Analyzes RNA degradation patterns across species or environmental conditions to understand evolutionary conservation and differences in regulatory mechanisms.",
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"Stress and Environmental Responses- Examines changes in RNA stability under stress conditions, shedding light on the molecular mechanisms of stress adaptation and survival strategies."
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];
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return (
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<ApplicationsLayout
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title="Applications of Degradome Sequencing"
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applicationItems={applicationItems}
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/>
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);
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};
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export default DegradomeApplications;
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// app/rna-sequencing/degradome-sequencing/components/DegradomeIntroduction.jsx
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import IntroductionLayout from '../../../components/shared/IntroductionLayout';
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const DegradomeIntroduction = () => {
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const contentItems = [
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"Degradome sequencing, also called Parallel Analysis of RNA Ends (PARE), is a high-throughput technique to analyze RNA degradation products, uncovering RNA stability, miRNA targets, and regulatory networks.",
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"Degradome Sequencing Workflow combines next-generation sequencing (NGS) with a modified 5' RACE method, involving RNA extraction, adapter ligation to capture degradation products, cDNA synthesis, and high-throughput sequencing to map RNA cleavage sites.",
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"Bioinformatics analysis for Degradome Sequencing include data preprocessing, aligning reads to reference genomes, identifying miRNA/ta-siRNA cleavage sites, and quantifying cleaved targets, revealing RNA decay dynamics and regulatory mechanisms.",
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"The method facilitates the discovery of novel miRNAs and ta-siRNAs, studies on miRNA-mediated gene regulation, and comparative RNA decay analysis across species, contributing to a comprehensive understanding of RNA life cycles."
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];
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return (
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<IntroductionLayout
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title="Introduction and Workflow"
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contentItems={contentItems}
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badgeSubtext="Brochure from Degradome sequencing"
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useParagraphs={true}
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/>
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);
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};
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export default DegradomeIntroduction;
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// DegradomeSpecifications.jsx
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import Link from 'next/link';
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import SpecificationsLayout from '../../../components/shared/SpecificationsLayout';
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const DegradomeSpecifications = () => {
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const specificationItems = [
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{
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icon: "/images/homepage-2/NGS-Icons-45.svg",
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title: "Sample Requirement",
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renderContent: () => (
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<div>
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<div className="mb-4">
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<p className="text-gray-600">Sample type: Total RNA without degradation or DNA contamination</p>
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<p className="text-gray-600">Total RNA≥ 20 μg, Minimum Quantity: 15 μg, Concentration≥ 100 ng/µL</p>
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</div>
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<div className="mt-4 text-sm">
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<strong>
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Please refer to{' '}
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<Link href="/sample-submission-guidelines" className="text-teal-600 underline hover:text-teal-700">
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sample submission guidelines
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</Link>
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{' '}or{' '}
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<Link href="/contact" className="text-teal-600 underline hover:text-teal-700">
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Contact Us!
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</Link>
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</strong>
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</div>
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</div>
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)
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},
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{
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icon: "/images/homepage-1/service/Advantages-NGS-Icons-20.svg",
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title: "Sequencing Platform",
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content: "Illumina NovaSeq 6000/ NovaSeq X"
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},
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{
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icon: "/images/service/social-support.png",
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title: "Deliverables",
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renderContent: () => (
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<ul className="list-disc pl-5 space-y-2 text-gray-600 text-start">
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<li>The original sequencing data</li>
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<li>Experimental results</li>
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<li>Bioinformatics and Data analysis report</li>
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<li>Details of Degradome Sequencing (customizable)</li>
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</ul>
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)
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}
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];
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return (
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<SpecificationsLayout
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title="Service Specifications"
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specificationItems={specificationItems}
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/>
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);
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};
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export default DegradomeSpecifications;
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27
app/rna-sequencing/degradome-sequencing/page.js
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27
app/rna-sequencing/degradome-sequencing/page.js
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import TitleBar from '../../components/shared/TitleBar';
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import DegradomeIntroduction from './components/DegradomeIntroduction';
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import DegradomeAdvantages from './components/DegradomeAdvantages';
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import DegradomeApplications from './components/DegradomeApplications';
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import DegradomeSpecifications from './components/DegradomeSpecifications';
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import PageLayout from '../../components/Layout/PageLayout';
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export default function DegradomeSequencingPage() {
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const breadcrumbs = [
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{ label: 'Home', href: '/', current: false },
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{ label: 'RNA Sequencing', href: '/rna-sequencing', current: false },
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{ label: 'Degradome Sequencing', href: '#', current: true }
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];
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return (
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<PageLayout fixedHeader={true}>
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<TitleBar
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title="Degradome Sequencing"
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breadcrumbs={breadcrumbs}
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/>
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<DegradomeIntroduction />
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<DegradomeAdvantages />
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<DegradomeApplications />
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<DegradomeSpecifications />
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</PageLayout>
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);
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}
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