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// MRNAAdvantages.jsx
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import AdvantagesLayout from '../../../components/shared/AdvantagesLayout';
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const MRNAAdvantages = () => {
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const advantageItems = [
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"mRNA-Seq provides a targeted analysis of gene expression specific to protein-coding regions, making it ideal for studies focused on gene regulation, protein synthesis, and disease-related expression changes.",
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"mRNA-Seq focuses on mRNA enrichment, which minimizes the impact of non-coding RNAs and enhances sensitivity for detecting gene expression changes.",
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"Focusing solely on coding regions, mRNA-Seq does not require rRNA-specific probes (Costly) for ribosomal RNA removal and requires comparatively lesser data, making it a more cost-effective alternative to Whole Transcriptome Sequencing.",
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"With a more targeted dataset, the bioinformatics analysis of mRNA-Seq is often simpler and faster, focusing on coding genes without the complexity introduced by non-coding RNAs."
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];
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return (
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<AdvantagesLayout
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title="Advantages of mRNA Sequencing (mRNA-Seq) over Whole Transcriptome Sequencing"
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advantageItems={advantageItems}
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/>
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);
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};
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export default MRNAAdvantages;
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// MRNAApplications.jsx
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import ApplicationsLayout from '../../../components/shared/ApplicationsLayout';
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const MRNAApplications = () => {
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const applicationItems = [
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"mRNA sequencing is widely utilized across plants, animals, microbes, and humans.",
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"Developmental Biology- Studies gene expression patterns during different stages of development, helping to unravel the mechanisms of cell differentiation and tissue formation.",
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"Disease Mechanisms- Uncovers mRNA expression profiles associated with various diseases, including genetic disorders and autoimmune conditions, to identify potential diagnostic markers and therapeutic targets.",
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"Drug Discovery- Analyzes mRNA expression changes in response to drug treatments, providing insights into drug mechanisms of action and helping to identify potential drug candidates.",
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"Functional Genomics- Provides a comprehensive view of gene expression across different conditions, facilitating the study of gene functions and regulatory networks.",
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"Comparative Genomics- Compares mRNA expression profiles across different species or environmental conditions to understand evolutionary differences and functional conservation.",
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"Toxicology- Assesses changes in gene expression in response to toxic substances, aiding in the identification of biomarkers for toxicity and understanding the impact of chemicals on biological systems."
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];
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return (
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<ApplicationsLayout
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title="Applications of mRNA Sequencing"
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applicationItems={applicationItems}
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/>
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);
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};
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export default MRNAApplications;
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// app/rna-sequencing/mrna-sequencing/components/MRNAIntroduction.jsx
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import IntroductionLayout from '../../../components/shared/IntroductionLayout';
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const MRNAIntroduction = () => {
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const contentItems = [
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"Messenger RNA-Seq (mRNA-Seq) is a focused RNA-Seq method that targets polyadenylated (poly-A) transcripts, which comprise only about 1-2% of the transcriptome, mainly representing coding genes. By enriching these poly-A-tailed RNAs, mRNA-Seq offers a precise snapshot of gene expression, capturing the complete range of mRNA transcripts within a sample and enabling detailed analysis of gene activity across various conditions.",
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"mRNA-seq workflow typically includes RNA extraction, enrichment or depletion of mRNA, library preparation, and high-throughput sequencing. This workflow ensures precise and efficient capture of mRNA transcripts for thorough downstream analysis.",
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"Bioinformatics analysis for mRNA-seq involves several key steps: data preprocessing, alignment to a reference genome, quantifying gene expression levels, and advanced downstream analyses such as differential expression, pathway enrichment, and gene ontology analysis.",
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"Widely applicable across diverse research fields, including human, animal, and plant studies, providing profound insights into genetic landscapes and contributing to our understanding of gene function and regulation."
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];
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return (
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<IntroductionLayout
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title="Introduction and Workflow"
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contentItems={contentItems}
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imageUrl="https://stock.adobe.com/in/images/messenger-rna-or-mrna-strand-3d-rendering-illustration-with-copy-space-genetics-science-medical-research-genome-replication-concepts/404350568"
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imageAlt="mRNA Sequencing Workflow"
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useParagraphs={true}
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/>
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);
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};
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export default MRNAIntroduction;
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// MRNASpecifications.jsx
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import Link from 'next/link';
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import SpecificationsLayout from '../../../components/shared/SpecificationsLayout';
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const MRNASpecifications = () => {
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const specificationItems = [
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{
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icon: "/images/homepage-2/NGS-Icons-45.svg",
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title: "Sample Requirement",
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renderContent: () => (
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<div>
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<div className="mb-4">
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<p className="text-gray-600">Total RNA ≥ 2 μg, Minimum Quantity: 500 ng, Concentration≥ 50 ng/µL</p>
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<p className="text-gray-600">Cells, Tissue and other samples</p>
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</div>
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<div className="mt-4 text-sm">
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<strong>
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Please refer to{' '}
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<Link href="/sample-submission-guidelines" className="text-teal-600 underline hover:text-teal-700">
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sample submission guidelines
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</Link>
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{' '}or{' '}
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<Link href="/contact" className="text-teal-600 underline hover:text-teal-700">
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Contact Us!
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</Link>
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</strong>
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</div>
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</div>
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)
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},
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{
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icon: "/images/homepage-1/service/Advantages-NGS-Icons-20.svg",
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title: "Sequencing Platform",
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content: "Illumina NovaSeq 6000/ NovaSeq X"
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},
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{
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icon: "/images/service/social-support.png",
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title: "Deliverables",
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renderContent: () => (
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<ul className="list-disc pl-5 space-y-2 text-gray-600 text-start">
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<li>The original sequencing data</li>
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<li>Experimental results</li>
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<li>Bioinformatics and Data analysis report</li>
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<li>Details of mRNA Sequencing (customizable)</li>
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</ul>
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)
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}
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];
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return (
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<SpecificationsLayout
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title="Service Specifications"
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specificationItems={specificationItems}
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/>
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);
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};
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export default MRNASpecifications;
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28
app/rna-sequencing/mrna-sequencing/page.js
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28
app/rna-sequencing/mrna-sequencing/page.js
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// app/rna-sequencing/mrna-sequencing/page.js
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import TitleBar from '../../components/shared/TitleBar';
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import MRNAIntroduction from './components/MRNAIntroduction';
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import MRNAAdvantages from './components/MRNAAdvantages';
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import MRNAApplications from './components/MRNAApplications';
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import MRNASpecifications from './components/MRNASpecifications';
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import PageLayout from '../../components/Layout/PageLayout';
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export default function MRNASequencingPage() {
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const breadcrumbs = [
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{ label: 'Home', href: '/', current: false },
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{ label: 'RNA Sequencing', href: '/rna-sequencing', current: false },
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{ label: 'mRNA Sequencing', href: '#', current: true }
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];
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return (
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<PageLayout fixedHeader={true}>
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<TitleBar
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title="mRNA Sequencing"
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breadcrumbs={breadcrumbs}
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/>
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<MRNAIntroduction />
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<MRNAAdvantages />
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<MRNAApplications />
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<MRNASpecifications />
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</PageLayout>
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);
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}
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