import React from 'react';
const GeneralGuidelines = () => {
return (
General Guidelines
-
Please complete the Sample Initiation Form (SIF), ensuring that the
sample names on the form match the labels on the sample tubes. We also
request that you send an electronic copy of the form and any required QC
data via email.
-
Each tube should be labeled on the lid with a maximum of 4-6
alphanumeric characters (e.g., 4B0001). Use a black permanent marker to
write sample names on the top and side of each tube. Avoid writing
directly on the tube wall or cover with an oil pen.
-
DNA can be submitted in DNase-free water, Elution Buffer, or 10mM Tris
pH 8.0. DNA samples should have an OD260/280 ratio as close to 1.8~2.0
as possible. All DNA should be RNase-treated and free from degradation
or contamination. Ship with ice packs. The total amount of DNA required
depends on the specific application.
-
RNA can be submitted in RNase-free water, RNA Stabilization Reagent, or
10mM Tris pH 8.0. All total RNA samples should be DNA-free, with an OD
A260/A280 ratio ≥ 1.8, A260/230 ratio ≥ 1.8, and a RIN ≥ 6. Ship with
dry ice. The total amount of RNA required depends on the specific
application. For Long Read Sequencing, RNA samples should have a RIN ≥
8.
-
The listed concentrations should be determined by fluorometry (e.g.,
PicoGreen/Qubit/RiboGreen). If using spectrophotometry (e.g., Nanodrop),
increase concentrations by approximately twofold.
-
The quality inspection method for the sizes and concentrations of the
Ready To Run Library is Qubit and Agilent Bioanalyzer.
);
};
export default GeneralGuidelines;